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Slit-lamp picture of the evolution of Acanthamoeba keratitis. Panel 1: cornea at the first visit. Note the ring infiltrate. Panel 2: corneal infiltrate did not improve following a PHMB-desomedine regimen every 1 h after 3 months. Panel 3: the corneal ulcer showed no signs of improvement at 2 months after therapy switch to hexamidine and chlorhexidine every 1 h. Panel 4: the corneal ulcer finally receded, leaving a vascularized leucoma 4 months following combined double-biguanide PHMB-chlorhexidine treatment. Note that the conjunctival hyperemia has also resolved. Panel 5: perforating corneal graft remained transparent 7 months after transplantation and no sign of recurrence was observed.